تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Gelsolin induces colorectal tumor cell invasion via modulation of the urokinase-type plasminogen activator cascade
Gelsolin induces colorectal tumor cell invasion via modulation of the urokinase-type plasminogen activator cascade
 
لغة الوثيقة : الانجليزية 
المستخلص : Gelsolin is a cytoskeletal protein which participates in actin filament dynamics and promotes cell motility and plasticity. Although initially regarded as a tumor suppressor, gelsolin expression in certain tumors correlates with poor prognosis and therapy-resistance. In vitro, gelsolin has anti-apoptotic and pro-migratory functions and is critical for invasion of some types of tumor cells. We found that gelsolin was highly expressed at tumor borders infiltrating into adjacent liver tissues, as examined by immunohistochemistry. Although gelsolin contributes to lamellipodia formation in migrating cells, the mechanisms by which it induces tumor invasion are unclear. Gelsolin's influence on the invasive activity of colorectal cancer cells was investigated using overexpression and small interfering RNA knockdown. We show that gelsolin is required for invasion of colorectal cancer cells through matrigel. Microarray analysis and quantitative PCR indicate that gelsolin overexpression induces the upregulation of invasion-promoting genes in colorectal cancer cells, including the matrix-degrading urokinase-type plasminogen activator (uPA). Conversely, gelsolin knockdown reduces uPA levels, as well as uPA secretion. The enhanced invasiveness of gelsolin-overexpressing cells was attenuated by treatment with function-blocking antibodies to either uPA or its receptor uPAR, indicating that uPA/uPAR activity is crucial for gelsolin-dependent invasion. In summary, our data reveals novel functions of gelsolin in colorectal tumor cell invasion through its modulation of the uPA/uPAR cascade, with potentially important roles in colorectal tumor dissemination to metastatic sites. 
ردمد : 1932-6203 
اسم الدورية : PloS one 
المجلد : 7 
العدد : 8 
سنة النشر : 1433 هـ
2012 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Tuesday, March 8, 2016 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Jingli ZhuoZhuo, Jingli باحث رئيسي  
Ee Hong TanTan, Ee Hong باحث مشارك  
Benedict YanYan, Benedict باحث مشارك  
Lalchhandami TochhawngTochhawng, Lalchhandami باحث مشارك  
Manikandan JayapalJayapal, Manikandan باحث مشارك  
Shiuan KohKoh, Shiuan باحث مشارك  
Hwee Kee TayTay, Hwee Kee باحث مشارك  
Sutherland K MaciverMaciver, Sutherland K باحث مشارك  
Shing Chuan HooiHooi, Shing Chuan باحث مشارك  
Manuel Salto-TellezSalto-Tellez, Manuel باحث مشارك  

الملفات

اسم الملفالنوعالوصف
 38429.pdf pdf 

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