تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis
Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis
 
لغة الوثيقة : الانجليزية 
المستخلص : The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. 
ردمد : 1359-5113 
اسم الدورية : Process Biochemistry 
المجلد : 74 
العدد : 12 
سنة النشر : 1433 هـ
2012 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Sunday, April 3, 2016 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Shakeel Ahmed AnsariAnsari, Shakeel Ahmedباحث رئيسي  
Rukhsana SatarSatar, Rukhsana باحث مشارك  
Fahad AlamAlam, Fahad باحث مشارك  
Mohammed Husein AlqahtaniAlqahtani, Mohammed Huseinباحث مشارك  
Adeel Gulzar ChaudharyChaudhary, Adeel Gulzarباحث مشارك  
Muhammad Imran NaseerNaseer, Muhammad Imranباحث مشارك  
Sajjad KarimKarim, Sajjad باحث مشارك  
Ishfaq Ahmed SheikhSheikh, Ishfaq Ahmedباحث مشارك  

الملفات

اسم الملفالنوعالوصف
 39260.pdf pdf 

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