تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Novel allergic asthma model demonstrates ST2-dependent dendritic cell targeting by cypress pollen
Novel allergic asthma model demonstrates ST2-dependent dendritic cell targeting by cypress pollen
 
لغة الوثيقة : الانجليزية 
المستخلص : Abstract BACKGROUND: Cypress pollen causes respiratory syndromes with different grades of severity, including asthma. IL-33, its receptor ST2, and dendritic cells (DCs) have been implicated in human respiratory allergy. OBJECTIVE: We sought to define a new mouse model of allergy to cypress pollen that recapitulates clinical parameters in allergic patients and to evaluate the implications of DCs and the IL-33/ST2 pathway in this pathology. METHODS: BALB/c mice, either wild-type or ST2 deficient (ST2(-/-)), were sensitized and challenged with the Cupressus arizonica major allergen nCup a 1. Local and systemic allergic responses were evaluated. Pulmonary cells were characterized by means of flow cytometry. DCs were stimulated with nCup a 1 and tested for their biological response to IL-33 in coculture assays. RESULTS: nCup a 1 causes a respiratory syndrome closely resembling human pollinosis in BALB/c mice. nCup a 1-treated mice exhibit the hallmarks of allergic pathology associated with pulmonary infiltration of eosinophils, T cells, and DCs and a dominant TH2-type immune response. IL-33 levels were increased in lungs and sera of nCup a 1-treated mice and in subjects with cypress allergy. The allergen-specific reaction was markedly reduced in ST2(-/-) mice, which showed fewer infiltrating eosinophils, T cells, and DCs in the lungs. Finally, stimulation of DCs with nCup a 1 resulted in ST2 upregulation that endowed DCs with increased ability to respond to IL-33-mediated differentiation of IL-5- and IL-13-producing CD4 T cells. CONCLUSIONS: Our findings define a novel preclinical model of allergy to cypress pollen and provide the first evidence of a functionally relevant linkage between pollen allergens and TH2-polarizing activity by DCs through IL-33/ST2. Copyright © 2013 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved. KEYWORDS: BAL; Bronchoalveolar lavage; C(T); Cycle threshold; DC; Dendritic cell; FITC; Fluorescein isothiocyanate; HPRT; Hypoxanthine phosphoribosyltransferase; IL-33; MLN; Mediastinal lymph node; OX40 ligand; OX40L; ST2; T cells; TSLP; Thymic stromal lymphopoietin; WT; Wild-type; allergic asthma; cypress pollen; dendritic cells; eosinophils; mouse model 
ردمد : 0091-6749 
اسم الدورية : Journal of Allergy and Clinical Immunology 
المجلد : 132 
العدد : 3 
سنة النشر : 1434 هـ
2013 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Thursday, April 7, 2016 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Lucia GabrieleGabriele, Lucia باحث رئيسي  
Giovanna SchiavoniSchiavoni, Giovanna باحث مشارك  
Fabrizio MatteiMattei, Fabrizio باحث مشارك  
Massimo SanchezSanchez, Massimo باحث مشارك  
Paola SestiliSestili, Paola باحث مشارك  
Cinzia ButteroniButteroni, Cinzia باحث مشارك  
Rita BusinaroBusinaro, Rita باحث مشارك  
Ananda MirchandaniMirchandani, Ananda باحث مشارك  
Wanda NiedbalaNiedbala, Wanda باحث مشارك  
Foo Y LiewLiew, Foo Y باحث مشارك  

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