تفاصيل الوثيقة
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نوع الوثيقة |
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مقال في مجلة دورية |
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عنوان الوثيقة |
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Microarray Expression Data Identify DCC as a Candidate Gene for Early Meningioma Progression Microarray Expression Data Identify DCC as a Candidate Gene for Early Meningioma Progression |
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لغة الوثيقة |
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الانجليزية |
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المستخلص |
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Meningiomas are the most common primary brain tumors bearing in a minority of cases an aggressive phenotype. Although meningiomas are stratified according to their histology and clinical behavior, the underlying molecular genetics predicting aggressiveness are not thoroughly understood. We performed whole transcript expression profiling in 10 grade I and four grade II meningiomas, three of which invaded the brain. Microarray expression analysis identified deleted in colorectal cancer (DCC) as a differentially expressed gene (DEG) enabling us to cluster meningiomas into DCC low expression (3 grade I and 3 grade II tumors), DCC medium expression (2 grade I and 1 grade II tumors), and DCC high expression (5 grade I tumors) groups. Comparison between the DCC low expression and DCC high expression groups resulted in 416 DEGs (p-value < 0.05; fold change > 2). The most significantly downregulated genes in the DCC low expression group comprised DCC, phosphodiesterase 1C (PDE1C), calmodulin-dependent 70kDa olfactomedin 2 (OLFM2), glutathione S-transferase mu 5 (GSTM5), phosphotyrosine interaction domain containing 1 (PID1), sema domain, transmembrane domain (TM) and cytoplasmic domain, (semaphorin) 6D (SEMA6D), and indolethylamine N-methyltransferase (INMT). The most significantly upregulated genes comprised chromosome 5 open reading frame 63 (C5orf63), homeodomain interacting protein kinase 2 (HIPK2), and basic helix-loop-helix family, member e40 (BHLHE40). Biofunctional analysis identified as predicted top upstream regulators beta-estradiol, TGFB1, Tgf beta complex, LY294002, and dexamethasone and as predicted top regulator effectors NFkB, PIK3R1, and CREBBP. The microarray expression data served also for a comparison between meningiomas from female and male patients and for a comparison between brain invasive and non-invasive meningiomas resulting in a number of significant DEGs and related biofunctions. In conclusion, based on its expression levels, DCC may constitute a valid biomarker to identify those benign meningiomas at risk for progression. |
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ردمد |
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1932-6203 |
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اسم الدورية |
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PLoS ONE |
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المجلد |
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11 |
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العدد |
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4 |
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سنة النشر |
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1437 هـ
2016 م |
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نوع المقالة |
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مقالة علمية |
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تاريخ الاضافة على الموقع |
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Wednesday, October 5, 2016 |
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الباحثون
| Hans-Juergen Schulten | Schulten, Hans-Juergen | باحث رئيسي | دكتوراه | |
| Deema Hussein | Hussein, Deema | باحث | | |
| Fatima Al-Adwani | Al-Adwani, Fatima | باحث | | |
| Sajjad Karim | Karim, Sajjad | باحث | دكتوراه | |
| Jaudah Al-Maghrabi | Al-Maghrabi, Jaudah | باحث | دكتوراه | |
| Mona Al-Sharif | Al-Sharif, Mona | باحث | | |
| Awatif Jamal | Jamal, Awatif | باحث | | |
| Fahad Al-GhamdI | Al-GhamdI, Fahad | باحث | | |
| Saleh Baeesa | Baeesa, Saleh | باحث | | |
| Mohammed Bangash | Bangash, Mohammed | باحث | | |
| Adeel Chaudhary | Chaudhary, Adeel | باحث | دكتوراه | |
| Mohammed Al-Qahtani | Al-Qahtani, Mohammed | باحث | | |
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الرجوع إلى صفحة الأبحاث
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